The Mechanism of Action of a Human Papilloma Virus Oncoprotein

The Mechanism of Action of a Human Papilloma Virus Oncoprotein

Source: Imai, Y., Y. Matsushima, T. Sugimura, M. Terada. 1991. Purification and characterization of human papillomavirus type 16 E7 protein with preferential binding capacity to the underphosphorylated form of retinoblastoma gene product. Journal of Virology 65(9): 4966–4972.

Corresponding chapter(s) in the textbook: Chapter 19 (and 17)Review the following terms before working on the problem: human papilloma virus, [35S]methionine labeling, [32P]phosphate labeling, phorbol ester, retinoblastoma protein, immunoprecipitation, SDS-polyacrylamide gel electrophoresis, autoradiography, pull-down assay, Western blotting

Read the paper and answer the questions below that refers to the data described in Figures 4 and 5 of the paper.

Be prepared to discuss the other experiments described, in class.

Experiment

Human papilloma virus strain 16 (HPV-16) is one of the causative agents of cervical cancer in women. The purpose of the research presented in the figure was to study the mechanism of action of the E7 oncoprotein of HPV-16.

Graph A shows the results of a preliminary experiment. Cells of a human leukemia cell line were cultured without (samples 1 and 3) or in the presence of phorbol ester (samples 2 and 4) and concurrently labeled with [35S]methionine (samples 1 and 2) or [32P]phosphate (samples 3 and 4). (Note: Phorbol ester stimulates protein kinase C.) Cell extracts were immunoprecipitated with an antibody specific for the retinoblastoma protein (anti-RB). SDS-polyacrylamide gel electrophoresis was performed, followed by autoradiography.

Graph B shows the results of the experiment designed to analyze the function of the E7 protein. Human leukemia cells were cultured in the absence (samples 1 to 3) or presence of phorbol ester (samples 4 to 6). Cell extracts were prepared, and aliquots were incubated with agarose beads to which E7 protein molecules had been covalently attached. After incubation, the beads were sedimented by centrifugation, and Western blot analysis was performed using the anti-RB antibody on the supernatants (S; samples 2 and 5), pellets (P; samples 3 and 6), or total cell extracts (T; samples 1 and 4).

Figure

A) Analysis of RB-immunoprecipitates from human leukemia cell extracts

B) Analysis of RB and E7 protein interactions by pull-down assay (T: total cell extract; S: supernatant; P: pellet)

Questions

1. What was the goal of [35S]methionine and [32P]phosphate labeling?

2. What do bands a and b represent?

3. What does the state of RB protein (graph A) tell you about the cell cycle conditions of the leukemia cells?

4. How did phorbol ester treatment affect the synthesis and phosphorylation of the RB protein?

5. How would the effect of phorbol ester treatment affect the leukemia cell culture?

6. What phenomenon was studied in the experiment that produced graph B?

7. Which form of the RB protein is affected by the E7 oncoprotein?

8. What effect would expression of the E7 protein in the leukemia cells have?

9. Summarize the mechanism of action of the E7 oncoprotein.

© 2016 Sinauer Associates, Inc.

 
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Rule out malingering and factitious disorder. (Are the symptoms genuine?

 
Unit 2 – Discussion 1 & 2? $30.00 Due (Monday) 7/16/2018.
Unit 2 Study 3
Forming a Differential Diagnosis
Counselors of children and adolescents typically receive informal information about their client before the first meeting. Someone has become concerned enough about the young person to suggest counseling. Counselors use this background information to develop questions for themselves that they try to answer in their first interview with a child. The answers lead the counselor through a process called differential diagnosis, whereby various plausible alternatives are ruled out to form an initial diagnosis. The initial diagnosis is the basis for the initial treatment plan. The initial diagnosis and treatment plan may be changed later as more facts come to light, but they are very important because they offer a counselor a place to start. Your task for this assignment is to develop a differential diagnosis.
Choose either the case of Luis, the child, or the case of Crystal, the teen. Decide upon your diagnosis by working through these six specific steps, as described in the DSM-5 Handbook of Differential Diagnosis, linked in Resources (see pages 1–16).
1. Rule out malingering and factitious disorder. (Are the symptoms genuine?)
2. Rule out substance etiology. (Are the symptoms a result of the consumption of drugs or alcohol?)
3. Rule out an etiological medical condition. (Is there a medical condition that explains the symptoms?)
4. Determine the specific primary disorder(s). (What appears to be the most accurate initial diagnosis?)
5. Differentiate Adjustment Disorder from the residual Other Specified or Unspecified Disorders. (Have the symptoms developed into a sufficiently maladaptive response meriting a primary disorder, or are they better described with one of these other diagnoses?)
6. Establish the boundary with “no mental disorder.” (Is a primary diagnosis merited due to “clinically significant” symptoms, or are the concerns better described as “Other Conditions that May Be a Focus of Clinical Attention“? Not all counseling involves treating a mental disorder.)
The Case of Luis
Luis is in the fourth grade. His mother is concerned that he may need counseling because he has not grown out of a fear he has had since he was young that is now interfering with developing peer friendships. Luis is afraid of vomiting in a public place. As you speak with him, you learn that when he was in kindergarten, he once got upset and cried so much that he vomited. His teacher was not particularly sympathetic and his parents could not be reached for a while, so he spent several hours with soiled clothes, feeling very ashamed and embarrassed. Subsequently, he became fearful that he might vomit again, with no reason or warning. His behaviors have become more restricted over the years, and now he will not eat if his family goes out to dinner because he fears the food might make him sick. He eats very little at school—just a few foods that he has decided are safe—and his peers have begun to tease him. If he eats something and starts worrying about vomiting, he soon begins to feel sick and often does vomit. So he has begun avoiding more and more social occasions; he declines invitations to birthday parties and sleepovers because he doesn’t want to refuse to eat (and risk being teased) but he is afraid that he will vomit if he does eat. His mother says she has always had trouble with a sensitive stomach so she is very sympathetic toward Luis’s fears. However, Luis’s pediatrician says he can find no medical problem with Luis.
Note: It is important to follow your protocol in exactly the order it is presented below.
Develop a differential diagnosis for Luis by reviewing the following DSM-5 Handbook for Differential Diagnosis Decision Trees and Tables:
o 2.13 Decision Tree for Anxiety.
o 2.15 Decision Tree for Avoidance Behavior.
o 2.17 Decision Tree for Somatic Complaints or Illness/Appearance Anxiety.
o 2.18 Decision Tree for Appetite Changes or Unusual Eating Behavior.
o 3.5.3 Specific Phobia.
o 3.5.4 Social Anxiety Disorder (social phobia).
o 3.10.1 Avoidant/Restrictive Food Intake Disorder.
The Case of Crystal
Crystal is a 14-year-old girl in the tenth grade. Crystal’s school counselor has contacted you in order to refer her due to concerns about recent changes in her behavior. Last year, when Crystal was in ninth grade, her grandmother had a fatal stroke. Crystal was very attached to her grandmother, as she had lived with her intermittently during childhood. Crystal’s teachers reported that she seemed extraordinarily sad about this loss; she lost interest in her studies and seemed very withdrawn, spending entire class periods with her head on her desk. This year, however, Crystal has been very irritable toward her teachers and has been caught fighting with other girls on several occasions over minor provocations. She has been suspended twice for this behavior. She was recently referred to a community health center, where no physical problems were found. When you talk with Crystal, she confirms all this but also tells you that she witnessed her grandmother’s death, and that her father blames her, saying Crystal “stressed her out.” Crystal says she hates her current family situation because her father plays favorites with her siblings and step-siblings, and admits to occasionally sneaking whiskey from her father’s stash when she feels very upset. She says she finds it difficult to concentrate in school. She feels very guilty about her grandmother, and says she sometimes wishes she could join her “on the other side.”
Develop a differential diagnosis for Crystal by reviewing the following DSM-5 Handbook for Differential Diagnosis Decision Trees and Tables:
o 2.1 Decision Tree for Poor School Performance.
o 2.15 Decision Tree for Behavioral Problems in a Child or Adolescent.
o 2.9 Decision Tree for Irritable Mood.
o 2.9 Decision Tree for Depressed Mood.
o 2.16 Decision Tree for Trauma or Psychosocial Stressors Involved in the Etiology.
o 3.4.1 Depressive Disorders.
o 3.4.4 Disruptive Mood Disorder.
o 3.14.1 Oppositional Defiant Disorder.
o 3.7.1 Trauma- and Stressor-Related Disorder.
o 3.15.1 Substance Use Disorders.
The differential diagnosis for your chosen case will be used in this unit’s discussion.
Resources
·Diagnosing Children and Adolescents
In your Discussion post, use the differential diagnosis you developed for your chosen case in this unit’s third study:
o Note your thoughts for each of the six steps, including your rationale for your initial diagnosis.
o What other diagnoses did you consider, but tentatively rule out?
o List three questions you could pursue in future sessions to be even surer of your diagnosis (by ruling out other potential diagnoses).
Resources

 
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How can I apply the Orem Theory as a nurse working in the intensive care unit, health and medicine homework help

How can I apply the Orem Theory as a nurse working in the intensive care unit, health and medicine homework help


Question descriptionNursing Theory Analysis Paper The purpose of this assignment is to describe, evaluate and discuss application of a nursing grand or mid-range theory. This assignment also provides the learner an opportunity to connect theory and research to nursing phenomena. Learners will develop an 8-10 page paper (excluding the title page and references) using APA style to address the elements listed below. Theory/Author Name and Background 1.Select a Grand or Mid-Range Theory that is appropriate to your practice setting. 2.Describe the theorist’s background in detail and discuss how their experiences have impacted the theory development. 3.Examine crucial references for the original and/or current work of the theorist and other authors writing about the selected theory. 4.Identify the phenomenon of concern or problems addressed by the theory. Theory Description 1.Explain whether the theory uses deductive, inductive or retroductive reasoning. Provide evidence to support your conclusion. 2.Describe the major concepts of the theory. How are they defined? (theoretically and/or operationally) Is the author consistent in the use of the concepts and other terms in the theory? 3.Interpret how the concepts are defined. Implicitly or explicitly? 4.Examine the relationships (propositions) among the major concepts. Evaluation 1.Identify explicit and implicit assumptions (values/beliefs) underlying the theory. On what assumptions does the theory build? 2.Examine if the theory has a description of the four metaparadigm concepts of nursing. If so, how are they explained in the theory? If the metapardigm is not explained, what elements do you see as relevant to the theory and why? 3.Discuss the clarity of the theory. Did it have lucidness and consistency? Application 1.Examine how the theory would guide nursing actions. 2.Describe specifically how you can use this theory in your area of nursing (Practice, Education, Informatics or Administration). APA Style/Format

 
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Research a health care organization or a network that spans several states within the U.S.

 

Details:
Research a health care organization or a network that spans several states within the U.S. (Example: United Healthcare, Vanguard, Banner Healthcare, etc.).
Harvard Business Review Online and Hoover’s Company Records, found in the GCU Library, are useful sources. You may also find pertinent information on your organization’s webpage.
Review “Singapore Airlines Case Study.”
Prepare a 1,000-1250-word paper that focuses on the organization or network you have selected.
Your essay should assess the readiness of the health care organization or network in addressing the health care needs of citizens in the next decade, and include a strategic plan that addresses issues pertaining to network growth, nurse staffing, resource management, and patient satisfaction. Provide a comparison to the health care organization or network and the Singapore Airlines. Include any cultural issues that may influence the practices listed above.
Prepare this assignment according to the APA guidelines found in the APA Style Guide, located in the Student Success Center. An abstract is not required.
This assignment uses a rubric. Please review the rubric prior to beginning the assignment to become familiar with the expectations for successful completion.
You are required to submit this assignment to Turnitin. Please refer to the directions in the Student Success Center.
 
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Survey of patient's experiences

Discussion

Directions

  1. Review information found on the following website related to hospital compare http://www.cms.gov/Medicare/Quality-Initiatives-Patient-Assessment-Instruments/HospitalQualityInits/HospitalCompare.html (Links to an external site.)Links to an external site.
  2. Read the information carefully and then locate the following website. http://www.medicare.gov/hospitalcompare (Links to an external site.)Links to an external site.
  3. Search for hospitals within a 50-mile radius of the community where you are working or had your prelicensure clinical experiences. Type in your zip code. Select your hospital and two others. Select hospitals/facilities and choose compare. If you live in a remote area and there are no hospitals listed within a 50-mile radius, select a zip code for a family member or a close friend who does not live near you. The idea is to review comparative data.
  4. Click on the following topics to learn more.
    1. Survey of patient’s experiences
    2. Timely and effective care (focus your search on two of the conditions that apply to services provided at the hospitals)
    3. Readmissions, complications, and deaths
  5. Carefully read the information provided.
  6. Develop a PowerPoint slideshow consisting of 8-10 slides. Include the following, keeping in mind what all this data means.
    1. Title slide with information pertinent to the course.
    2. List reasons to recommend the website Hospital Compare to consumers (patients).
    3. List reasons to recommend the website Hospital Compare to staff who may seek employment.
  7. For the slides below, clearly label the name of each hospital:
    1. Summarize patient experiences data for each hospital.
    2. Summarize timely and effective care data for two conditions.
    3. Summarize data for readmissions and complications and deaths.
    4. List recommendations for improving data for one selected facility.
    5. Summarize what you learned from this experience.
 
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Identify and discuss the duties and responsibilities of a pharmacist toward a patient

Anonymous. (2015). Enforcement procedures for the Occupational Therapy Code of Ethics. The American Journal of Occupational Therapy, 69(Suppl. 3) 1-13. Retrieved from the Trident Online Library.Baker,

Anonymous. (2015). Enforcement procedures for the Occupational Therapy Code of Ethics. The American Journal of Occupational Therapy, 69(Suppl. 3) 1-13. Retrieved from the Trident Online Library.

Baker, K. R. (2016) An obligation to society. Drug Topics, 160(10). Retrieved from the Trident Online Library.

Battie, R. N. (2015) Thriving through trust: Ethical practice, quality care. AORN Journal, 101(5). 505-507. Retrieved from the Trident Online Library.

Chiarello, E. (2015) The War on Drugs comes to the pharmacy counter: Frontline work in the shadow of discrepant institutional logics. Law and Social Inquiry, 40(1) 86-122. Retrieved from the Trident Online Library.

Divakar, D. D., Al Kheraif, A. A., Ramakrishnaiah, R., Al Zhrane, M., Al-Hazmi, A., and Parine, N. R. (2015). Ethics in dentistry an overview: Electronic search study. Revista Romana de Bioteca, 12(3). Retrieved from the Trident Online Library.

Grass, J. C. (2016). The Medicine Shoppe v. Loretta Lynch, et al.: Pharmacists and prescribing physicians are equally liable. The Health Lawyer, 28(3), 28-37. Retrieved from the Trident Online Library.

Limenti, A. E. (1999). The role of ethical principles in health care and the implications for ethical codes. Journal of Medical Ethics, 25. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC479265/pdf/jmedeth00006-0032.pdf

Yang, Y. T., Attanasio, L. B., & Kozhimannil, K. B. (2016). State scope of practice laws, nurse-midwifery workforce, and childbirth procedures and outcomes. Women’s Health Issues, 26(3) 262-267. Retrieved from the Trident Online Library.

Young, G., Hulcombe, J., Hurwood, A., & Nancarrow, S. (2015). The Queensland Health Ministerial Taskforce on health practitioners’ expanded scope of practice: Consultation findings. Australian Health Review, 39(3), 249-254. Retrieved from the Trident Online Library.

Optional Reading

American Clinical Social Work Association (n.d.) Code of Ethics. Retrieved from http://www.acswa.org/code-ethics/

American Society of Clinical Laboratory Science (n.d.) Code of Ethics. Retrieved from  http://www.ascls.org/about-us/code-of-ethics

International Board of Lactation Consultant Examiners (2015) Code of Professional Conduct. Retrieved from https://iblce.org/wp-content/uploads/2017/05/code-of-professional-conduct.pdf

National Association of Emergency Medical Technicians (2013). Code of Ethics. Retrieved from https://www.naemt.org/about-ems/emt-oath

**Homework Assignment

Read the article in the required reading, “The Medicine Shoppe v. Loretta Lynch, et al.: Pharmacists and prescribing physicians are equally liable” as well as the Pharmacists Code of Ethics at the following link:https://www.pharmacist.com/code-ethics

Discuss the following:

  1. Identify and discuss the duties and responsibilities of a pharmacist toward a patient.
  2. Based upon your research and assessment of the case, did the pharmacist perform the required duties and uphold patient rights?
  3. Is the pharmacy at fault? Why or why not?
  4. What impact can a pharmacist’s actions have on patients and community?
  5. What precautions can be taken to ensure these types of incidents by allied professionals don’t happen to others? Should there be more regulations in place?

Length: Submit a 3-page paper, using headings to correspond with each question in the assignment.

SLP Assignment Expectations

  1. Conduct additional research to gather sufficient information to support your writing. This should not be an opinion piece, but an analysis.
  2. Avoid the use of first person in your paper.
  3. Limit your response to a maximum of 3-5 pages.
  4. Support your paper with peer-reviewed articles and reliable sources, and use at least 3 references. For additional information on how to recognize peer-reviewed journals, see http://www.angelo.edu/services/library/handouts/peerrev.php and for evaluating internet sources:https://www.library.georgetown.edu/tutorials/research-guides/evaluating-internet-content
  5. You may use the following source to assist in your formatting your assignment: https://owl.english.purdue.edu/owl/resource/560/01/
 
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Compare your future role with one of the following: nurse educator; nurse leader; family nurse practitioner; acute care nurse practitioner; graduate nurse with an emphasis/specialty in public health, health care administration, business, or informatics; clinical nurse specialist; doctor of nursing practice

Details:

Advanced registered nurses work in highly collaborative environments and must collaborate with interdisciplinary teams in order to provide excellent patient care. Besides knowing the role and scope of one’s own practice, it is essential to understand the role and scope of other nurse specialties to ensure effective collaboration among nurses, the organization, and other professionals with whom advanced registered nurses regularly interact.
Use the “Nursing Roles Graphic Organizer Template” to differentiate how advanced registered nurse roles relate to and collaborate with different areas of nursing practice. Compare your future role with one of the following: nurse educator; nurse leader; family nurse practitioner; acute care nurse practitioner; graduate nurse with an emphasis/specialty in public health, health care administration, business, or informatics; clinical nurse specialist; doctor of nursing practice. Indicate in the appropriate columns on the template which roles you are comparing.
Make sure to compare the following areas of practice in your graphic organizer:

Ethics
Education
Leadership
Public Health
Health Care Administration
Informatics
Business/Finance
Specialty (e.g., Family, Acute Care)

Include any regulatory bodies or certification agencies that provide guidance or parameters on how these roles incorporate concepts into practice.
You are required to cite three to five sources to complete this assignment. Sources must be published within the last 5 years and appropriate for the assignment criteria and nursing content.
While APA style is not required for the body of this assignment, solid academic writing is expected, and documentation of sources should be presented using APA formatting guidelines, which can be found in the APA Style Guide, located in the Student Success Center.
This assignment uses a rubric. Please review the rubric prior to beginning the assignment to become familiar with the expectations for successful completion.
You are not required to submit this assignment to Turnitin.
Hello,
I will need a cover page, then the nurse graphic that I sent to you then the last the reference page where you willl provide the references that you used to do the work. As a previously sent on the assignment it needs to be at least 3 to 5 reference sources. Also my computer is a MacBook Air and I have pages as my program for writing so if you sent to me needs to open on this one. Also if possible at the end convert the file to PDF as the university website request PDF

 
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uggest one psychological research question that could be answered by each of the following types of statistical tests:

 

Assignment 1: Generating Research Questions for z-Tests and t-Tests

Suggest one psychological research question that could be answered by each of the following types of statistical tests:

  • z test
  • t test for independent samples, and
  • t test for dependent samples

Present the rationale for your selection. Be very specific in your presentation.
Write your initial response in 3–4 paragraphs. If you refer to research information, cite sources applying APA standards.

Assignment 1: Generating Research Questions for z-Tests and t-Tests

Suggest one psychological research question that could be answered by each of the following types of statistical tests:

  • z test
  • t test for independent samples, and
  • t test for dependent samples

Present the rationale for your selection. Be very specific in your presentation.
Write your initial response in 3–4 paragraphs. If you refer to research information, cite sources applying APA standards.

Make up at least ten (10) open-ended questions and ask them of a minimum of 5 adolescents. The questions could concern school, sex, food, use of time, occupation, plans for the future, etc. Record their answers as best you can and then write out your evaluation of the adolescents’ identity formation based on the information presented in this course.
 
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Discuss the benefits of expanding your network. What opportunities could this provide for your organization?

Discuss the benefits of expanding your network. What opportunities could this provide for your organization?

Option A: Hospitals frequently seek ways to ensure survival. Sometimes an adversarial climate cannot be avoided when the action that is being considered is controversial. For example, a hospital may seek to expand its market reach by opening an inpatient drug treatment facility in a small suburban township. The county where the township sits has seen a steady rise in heroin addiction, as well as an increase in hepatitis cases. As a manager, identify the three possible categories of clients within this new clientele network. Give at least one example of a client for each category and explain the reason for your decision.

Guided Response: Review several of your classmates’ posts. Provide a substantive response to at least two of your peers. Discuss the benefits of expanding your network. What opportunities could this provide for your organization?

Option B: In this increasingly difficult economic environment, many organizations have been forced to take drastic operational and systematic measures to stay afloat and maintain their position within the community. If you were a health care manager leading an organization in which its financial, political, and/or social position was threatened, what survival strategies would you employ? Please choose at least three strategies from your textbook to discuss and thoroughly explain the rationale for your choices.

QUESTION 2

Change is usually positive, but it can also be difficult to embrace at times. Employees can be resistant to change, while managers may feel that it is necessary for organizational stability. Discuss the challenge of change as it relates to health care organizations. What is the overall impact of change on the organization, its employees, and its constituents, and what role does the manager play as an agent of change? In addition to responding to these questions, review the examples of successful change in your textbook and choose two examples to discuss in greater detail. What did you learn from these examples? In each case, how did the individuals overcome the barriers to change, and what were the resulting benefits?


 

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Discuss in your own opinion the ethics of medical mistakes that are not fully disclosed.

Discuss in your own opinion the ethics of medical mistakes that are not fully disclosed.

Discuss in your own opinion the ethics of medical mistakes that are not fully disclosed. Need to address the legal and ethical environment that may prohibit full disclosure of medical mistakes to the patient. Include discussion about the healthcare trends that will focus attention toward an ethical culture of reporting medical mistakes.


 

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